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sgrna scaffold  (Addgene inc)


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    Addgene inc sgrna scaffold
    Sgrna Scaffold, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sgrna scaffold/product/Addgene inc
    Average 95 stars, based on 237 article reviews
    sgrna scaffold - by Bioz Stars, 2026-03
    95/100 stars

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    a Schematic of S protein and VLP-S2. b SDS-PAGE analysis of individual S2 antigens and VLP-S2s. Characterization by SDS-PAGE was performed twice using different preparations of each sample with similar results. c Representative dynamic light scattering characterization of S2 antigen, <t>MS2,</t> and VLP-S2 constructs. d S2P6 binding characterization of S2 antigens and VLP-S2 constructs (mean ± SD, n = 3), one assay with three technical replicates. e Represenative size exclusion chromography chromatogram of BtKY72 S2 and BtKY72 Tr. S2 antigens, both purified from transfection volumes of 90 mL. f Total yield of protein expected from 1 L of media, extrapolated from separate 50 mL volume expressions (mean ± SD, n = 3). g Melting temperature analysis of BtKY72 S2 and BtKY72 Tr. S2 antigens.
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    a Schematic of S protein and VLP-S2. b SDS-PAGE analysis of individual S2 antigens and VLP-S2s. Characterization by SDS-PAGE was performed twice using different preparations of each sample with similar results. c Representative dynamic light scattering characterization of S2 antigen, <t>MS2,</t> and VLP-S2 constructs. d S2P6 binding characterization of S2 antigens and VLP-S2 constructs (mean ± SD, n = 3), one assay with three technical replicates. e Represenative size exclusion chromography chromatogram of BtKY72 S2 and BtKY72 Tr. S2 antigens, both purified from transfection volumes of 90 mL. f Total yield of protein expected from 1 L of media, extrapolated from separate 50 mL volume expressions (mean ± SD, n = 3). g Melting temperature analysis of BtKY72 S2 and BtKY72 Tr. S2 antigens.
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    a Schematic of S protein and VLP-S2. b SDS-PAGE analysis of individual S2 antigens and VLP-S2s. Characterization by SDS-PAGE was performed twice using different preparations of each sample with similar results. c Representative dynamic light scattering characterization of S2 antigen, <t>MS2,</t> and VLP-S2 constructs. d S2P6 binding characterization of S2 antigens and VLP-S2 constructs (mean ± SD, n = 3), one assay with three technical replicates. e Represenative size exclusion chromography chromatogram of BtKY72 S2 and BtKY72 Tr. S2 antigens, both purified from transfection volumes of 90 mL. f Total yield of protein expected from 1 L of media, extrapolated from separate 50 mL volume expressions (mean ± SD, n = 3). g Melting temperature analysis of BtKY72 S2 and BtKY72 Tr. S2 antigens.
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    a Schematic of S protein and VLP-S2. b SDS-PAGE analysis of individual S2 antigens and VLP-S2s. Characterization by SDS-PAGE was performed twice using different preparations of each sample with similar results. c Representative dynamic light scattering characterization of S2 antigen, <t>MS2,</t> and VLP-S2 constructs. d S2P6 binding characterization of S2 antigens and VLP-S2 constructs (mean ± SD, n = 3), one assay with three technical replicates. e Represenative size exclusion chromography chromatogram of BtKY72 S2 and BtKY72 Tr. S2 antigens, both purified from transfection volumes of 90 mL. f Total yield of protein expected from 1 L of media, extrapolated from separate 50 mL volume expressions (mean ± SD, n = 3). g Melting temperature analysis of BtKY72 S2 and BtKY72 Tr. S2 antigens.
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    a Schematic of S protein and VLP-S2. b SDS-PAGE analysis of individual S2 antigens and VLP-S2s. Characterization by SDS-PAGE was performed twice using different preparations of each sample with similar results. c Representative dynamic light scattering characterization of S2 antigen, <t>MS2,</t> and VLP-S2 constructs. d S2P6 binding characterization of S2 antigens and VLP-S2 constructs (mean ± SD, n = 3), one assay with three technical replicates. e Represenative size exclusion chromography chromatogram of BtKY72 S2 and BtKY72 Tr. S2 antigens, both purified from transfection volumes of 90 mL. f Total yield of protein expected from 1 L of media, extrapolated from separate 50 mL volume expressions (mean ± SD, n = 3). g Melting temperature analysis of BtKY72 S2 and BtKY72 Tr. S2 antigens.
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    a Schematic of S protein and VLP-S2. b SDS-PAGE analysis of individual S2 antigens and VLP-S2s. Characterization by SDS-PAGE was performed twice using different preparations of each sample with similar results. c Representative dynamic light scattering characterization of S2 antigen, <t>MS2,</t> and VLP-S2 constructs. d S2P6 binding characterization of S2 antigens and VLP-S2 constructs (mean ± SD, n = 3), one assay with three technical replicates. e Represenative size exclusion chromography chromatogram of BtKY72 S2 and BtKY72 Tr. S2 antigens, both purified from transfection volumes of 90 mL. f Total yield of protein expected from 1 L of media, extrapolated from separate 50 mL volume expressions (mean ± SD, n = 3). g Melting temperature analysis of BtKY72 S2 and BtKY72 Tr. S2 antigens.
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    Image Search Results


    a Schematic of S protein and VLP-S2. b SDS-PAGE analysis of individual S2 antigens and VLP-S2s. Characterization by SDS-PAGE was performed twice using different preparations of each sample with similar results. c Representative dynamic light scattering characterization of S2 antigen, MS2, and VLP-S2 constructs. d S2P6 binding characterization of S2 antigens and VLP-S2 constructs (mean ± SD, n = 3), one assay with three technical replicates. e Represenative size exclusion chromography chromatogram of BtKY72 S2 and BtKY72 Tr. S2 antigens, both purified from transfection volumes of 90 mL. f Total yield of protein expected from 1 L of media, extrapolated from separate 50 mL volume expressions (mean ± SD, n = 3). g Melting temperature analysis of BtKY72 S2 and BtKY72 Tr. S2 antigens.

    Journal: NPJ Vaccines

    Article Title: Characterization and immunogenicity of nanoparticle vaccines based on Clade 2 and Clade 3 sarbecovirus S2 proteins

    doi: 10.1038/s41541-025-01333-4

    Figure Lengend Snippet: a Schematic of S protein and VLP-S2. b SDS-PAGE analysis of individual S2 antigens and VLP-S2s. Characterization by SDS-PAGE was performed twice using different preparations of each sample with similar results. c Representative dynamic light scattering characterization of S2 antigen, MS2, and VLP-S2 constructs. d S2P6 binding characterization of S2 antigens and VLP-S2 constructs (mean ± SD, n = 3), one assay with three technical replicates. e Represenative size exclusion chromography chromatogram of BtKY72 S2 and BtKY72 Tr. S2 antigens, both purified from transfection volumes of 90 mL. f Total yield of protein expected from 1 L of media, extrapolated from separate 50 mL volume expressions (mean ± SD, n = 3). g Melting temperature analysis of BtKY72 S2 and BtKY72 Tr. S2 antigens.

    Article Snippet: A plasmid encoding BirA biotin-protein ligase was co-transformed alongside the MS2 plasmid into BL21(DE3) competent E. coli (New England Biolabs) per the manufacturer’s protocol.

    Techniques: SDS Page, Construct, Binding Assay, Purification, Transfection